Guan Lab

Department of Computational Medicine & Bioinformatics
Wed, 02/08/2017 - 12:22 -- gyuanfan
TitleAnnotation of Alternatively Spliced Proteins and Transcripts with Protein-Folding Algorithms and Isoform-Level Functional Networks.
Publication TypeJournal Article
Year of Publication2017
AuthorsLi H, Zhang Y, Guan Y, Menon R, Omenn GS
JournalMethods Mol Biol.
Start Page415
Issue1558
Pagination415-436
Date Published02/2017
KeywordsFunctional prediction; Isoform network; Protein folding; Splice isoforms
Abstract

Tens of thousands of splice isoforms of proteins have been catalogued as predicted sequences from transcripts in humans and other species. Relatively few have been characterized biochemically or structurally. With the extensive development of protein bioinformatics, the characterization and modeling of isoform features, isoform functions, and isoform-level networks have advanced notably. Here we present applications of the I-TASSER family of algorithms for folding and functional predictions and the IsoFunc, MIsoMine, and Hisonet data resources for isoform-level analyses of network and pathway-based functional predictions and protein-protein interactions. Hopefully, predictions and insights from protein bioinformatics will stimulate many experimental validation studies.