Guan Lab

Department of Computational Medicine & Bioinformatics
Wed, 11/06/2013 - 10:20 -- gl_admin
TitleSystematic planning of genome-scale experiments in poorly studied species.
Publication TypeJournal Article
Year of Publication2010
AuthorsGuan Y, Dunham M, Caudy A, Troyanskaya O
JournalPLoS Comput Biol
Date Published2010 Mar
KeywordsChromosome Mapping, Computer Simulation, Gene Expression Profiling, Models, Biological, Oligonucleotide Array Sequence Analysis, Proteome, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Signal Transduction, Species Specificity

Genome-scale datasets have been used extensively in model organisms to screen for specific candidates or to predict functions for uncharacterized genes. However, despite the availability of extensive knowledge in model organisms, the planning of genome-scale experiments in poorly studied species is still based on the intuition of experts or heuristic trials. We propose that computational and systematic approaches can be applied to drive the experiment planning process in poorly studied species based on available data and knowledge in closely related model organisms. In this paper, we suggest a computational strategy for recommending genome-scale experiments based on their capability to interrogate diverse biological processes to enable protein function assignment. To this end, we use the data-rich functional genomics compendium of the model organism to quantify the accuracy of each dataset in predicting each specific biological process and the overlap in such coverage between different datasets. Our approach uses an optimized combination of these quantifications to recommend an ordered list of experiments for accurately annotating most proteins in the poorly studied related organisms to most biological processes, as well as a set of experiments that target each specific biological process. The effectiveness of this experiment- planning system is demonstrated for two related yeast species: the model organism Saccharomyces cerevisiae and the comparatively poorly studied Saccharomyces bayanus. Our system recommended a set of S. bayanus experiments based on an S. cerevisiae microarray data compendium. In silico evaluations estimate that less than 10% of the experiments could achieve similar functional coverage to the whole microarray compendium. This estimation was confirmed by performing the recommended experiments in S. bayanus, therefore significantly reducing the labor devoted to characterize the poorly studied genome. This experiment-planning framework could readily be adapted to the design of other types of large-scale experiments as well as other groups of organisms.


Featured in: Functional genomics: Learning to prioritize. Flintoft L. Nature Reviews Genetics 11, 315 (May 2010) | doi:10.1038/nrg2789

Alternate JournalPLoS Comput. Biol.
PubMed ID20221257
PubMed Central IDPMC2832676
Grant ListR01 GM071966 / GM / NIGMS NIH HHS / United States